Variation of protease activity in coconut kernel in relation to variety, nut maturity and season
Keywords:
Protease Activity, Seasonal Variation, Cocos nucifera, Coconut Kernel Development, BAPNA (N - Benzoyl DL- arginine p-nitroanilide)
Abstract
Studies on the presence and involvement of proteases in the development and maturation of endosperm of Cocos nucifera L. revealed changes in concentration of proteases with the age of the nut, variety and season. More BAPNA (Na - Benzoyl DL - arginine p-nitroanilide) hydrolysing activity. Was observed at the immature stage and more gelatinolytic activity at the mature stage of the coconut endosperm development. There was apparent difference between seasons, the highest protease activity was during winter months.
References
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Bradford, M.M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analyt. Biochem. 72: 248-254.
Laemmli, U.K. 1970. Cleavage of structural proteins during the assembly of the head of Bacteriophage T4. Nature 227: 680-685.
Manjula, C., Chempakam, B. and Rajagopal, V. 1993. Physiological and biochemical changes during the germination of coconut seeds. J. Plant. Crops 21 (supplement): 313-321.
Nagarajan, M. and Pandalai, K.M. 1963. Studies on the enzyme activity in the haustorium of germinating coconut. Indian Cocon. J. 17: 25-34.
Heussen, C. and Dowdle, E.B. 1980. Electrophoretic analysis of plasminogen activators in polyacrylamide gels containing sodium dodecyl sulfate and copolymerised substrates. Analyt. Biochem. 102: 196-202.
Published
2005-06-01
How to Cite
Panicker, L.M., Usha, R., & Mandan, C.N. (2005). Variation of protease activity in coconut kernel in relation to variety, nut maturity and season . CORD, 21(02), 34. https://doi.org/10.37833/cord.v21i02.413
Section
Articles
