DNA fingerprinting to distinguish the coconut type, San Ramon
Abstract
San Ramon, an introduced type of coconut from Philippines, has long been in Sri Lanka. San Ramon has the advantage of producing high copra, high oil, and high-quality timber and also seemed to be less variable for bunch characters. In addition, it is a type of coconut having an ability to withstand long droughts and also coconut mites. Due to these outstanding beneficial characters, San Ramon has been used as a parent for effecting various crosses to transmit the drought tolerance nature and high copra outturn, to the offspring. The physical structure of San Ramon palms is much comparable to tall type coconuts though there are some specific characters predominantly seen in favourable environments. On top of these circumstances, it is not easy to separate San Ramon from other Talls and virtually impossible to distinguish pure San Ramon from San Ramon crosses by means of only morphological markers. Therefore, a molecular finger-printing method was attempted to distinguish pure San Ramon using already developed 10 coconut SSR primers. The results indicate the possibility of distinguishing pure San Ramon from its crosses with the help of 2 coconut SSR primers, CNZ6 and CNZ44.
References
Bandaranayake, C.K. 2002. Genome mapping of Arabidopsis thaliana and Cocos nucifera. PhD thesis. The University of Birmingham, UK, 227 pp.
Bassam, B.J. and Caetano-Anolles, G. 1993. Silver staining of DNA in polyacrylamide gels. Appl. Biochem. Biotech. 42: 181-188.
CRI, 1987. Report of Genetics and Plant Breeding Division In: Annual Report of the Coconut Research Institute for 1987, CRISL, pp. 51-107.
Dayrit, C.S. 2000. Coconut oil in health and disease: Its and monolaurin’s potential as cure for HIV/AIDS. COCOTECH meeting 2000, Chennai, India.
Doyle, J.J. and Doyle, J.L. 1990. Isolation of plant DNA from fresh tissue. Focus 12: 13-15.
Enig, M.G. 1999. Functional foods for health promotion: Physiologic considerations, Experimental Biology 1999, Renaissance Washington Hotel, USA.
Enig, M.G. 2001. Coconut: In support of good health in the 21st century, APCC meeting 2001, Manila, Philippines.
Fernando, W.M.U. 1987. San Ramon, Big with Promise. Cocon. Bull. 4(1): 15.
Fernando, W.M.U. 1999. Genetic improvement of the coconut cultivar San Ramon. Cocos 13: 30-39.
Jayasekara, C., Ranasinghe, C.S. and Mathes, D.T. 1983. Screening for high yield and drought tolerance in coconut, pp. 209-217. In: Coconut Research and Development, N.M. Nayar (Ed.). Oxford IBH Publ. New Delhi, India.
Palomar, R.N. and Fernandez, V.A. 1994. Variability of some mechanical properties of coconut wood. Philippine J. Cocon. Stud. 19: 5-14
Perera, L., Russell, J.R., Provan, J. and Powell, W. 1999. Identification and characterization of microsatellite loci in coconut (Cocos nucifera L.) and the analysis of coconut populations in Sri Lanka. Molecular Ecol. 8: 335-346
Powell, W., Machray, G.C. and Provan, J. 1996. Polymorphism as revealed by simple sequence repeats. Trends in Plant Science 1: 215-222.
Ranasinghe, C.S. 1989. Physiological and biochemical characters of different varieties of coconut. In: Proc. Sri Lanka Assoc. for Advanc. Sci. 45: 55.
Rivera, R., Edwards, K.J., Barker, J.H.A., Arnold, G.M., Ayad, G., Hodgkin, T. and Karp, A. 1999. Isolation and characterization of polymorphic microsatellites in Cocos nucifera L. Genome 42: 668-675.
Singh, T.N., Paleg, L.G. and Aspinall, D. 1973. Stress metabolism. 1. Nitrogen metabolism and growth in the barley plant during water stress. Australian J. Biol. Sci. 26: 65-76.
Santos, G.A., Cano, S.B., Cruz, B.V., Ilagan, M.C. and Bahala, R.T. 1984. Coconut germplasm collection in the Philippines. Philippine J. Cocon. Stud. 6: 1-9.
Teulat, B., Aldam, C., Trehin, R., Lebrun, P., Barker, J.H.A., Arnold, G.M., Karp, A., Baudouin, L. and Rognon, F. 2000. An analysis of genetic diversity in coconut (Cocos nucifera) populations from across the geographic range using sequence-tagged microsatellites (SSRs) and AFLPs. Theor. Appl Genet. 100: 764-771.
