Cryopreservation of mature coconut embryos by desiccation method
Keywords:
Coconut (Cocos nucifera L.), Cryopreservation, Ddesiccation Pretreatment, Liquid Nitrogen, Mature Embryos, West Coast Tall
Abstract
Mature embryos of West Coast Tall variety of coconut could be cryopreserved after desiccation pretreatments and retrieved into plantlets. The desiccation pretreatment and optimum duration for dehydration of zygotic embryos were standardized. The maximum retrieval of healthy plantlets was obtained from the embryos subjected to 18 hours silica gel or 24 hours laminar airflow desiccation pretreatment. Irreversible damage caused by desiccation to the death of the shoot meristem was noticed when the water content reduced to 20%.
References
Assy-Bah, B. and Engelmann, F. 1992a. Cryopreservation of mature embryos of coconut (Cocos nucifera L.) and subsequent regeneration of planlets. Cryo-Letters 13:117-126.
Assy-Bah, B. and Engelmann, F. 1992b. Cryopreservation of immature embryos of coconut (Cocos nucifera L.) Cryo-Letters 13:67-74.
Chin, H.F., Krishnapillay, B. and Hor, Y.L. 1989. A note on the cryopreservation of embryos from young coconut (Coconut nucifera L.) var.Mawa. Pertanika 12: 183-186.
CPCRI, 2000. Annual Report - 2000. Central Plantation Crops Research Institute, Kasaragod. Kerala, India, pp.30-31.
Dumet, D., Engelmann, F., Chabrillange and Duval, Y. 1993. Cryopreservation of oil palm (Elaeis guineensis Jacq) somatic embryos involving a desiccation step. Plant Cell Reports: 12:352-355.
Engelmann, F. 1992. Cryopreservation of embryos, pp.281-290. In: Reproductive Biology and Plant Breeding, V. Dattee, C. Dumas and A. Gallais (Eds.) Springer Verlag Berlin.
Engelmann, F. 2000. Importance of cryopreservation for conservation of plant genetic resources. In: Cryopreservation of Tropical Plant Germplasm. Current Research Progress and Application. F. Engelmann and H. Takagi (Eds). JIRCAS, Japan/ IPGRI, Italy.
Gray, D.J., Conger, B.V, and Songstad, 1987. Desiccated quiescent somatic embryos of orchardgrass for use as synthetic seeds. In Vitro 23:29-33.
Grout, B.W.W , Shelton, K. and Pritchard, H.W. 1983. Orthodox behaviour of oil palm seed and cryopreservation of the excised embryo for genetic conservation Ann. Bot. 52:381-384.
Kim, Y.H and Janick, J. 1989 ABA and polyox-encapsulation of high humidity increases survival of desiccated somatic embryos of celery. Hort. Sci. 24:674-676.
Karun, A., Shivashankar, S., Sajini, K.K. and Saji, K.V. 1993 Field collection and in vitro germination of coconut embryos. J. Plant. Crops 21 (Suppl.): 291-294.
Karun, A., Sajini K.K. and Shivashankar, S. 1999. Embryo culture - A CPCRI protocol. Indian J. Horti. 56(4): 348-353.
Karun, A., Parthasarathy V.A., Kumaran P.M., Iyer, R.D. and Sajini, K.K. 2002. Coconut embryo culture - A protocol fpr germplasm collection Tech. Bull. 45 CPCRI, Kasaragod, India.
Niino, T. 1993. Cryopreservation of deciduous fruits and mulberry trees. In: Cryopreservation of Plant Genetic Resources A. Sakai (Ed) Ref No. 6 FARDA, Tokyo, pp.71-76.
Normah, M.N., Chin, H.F and Hor, Y.L. 1986. Desiccation and cryopreservation of embryonic axes of Hevea brasiliensis Mucl. Arg. Pertanika 9:299-303.
Pritchand, H.W. and Prendergast, F.G. 1986. Effect of desiccation on the in vitro viability of embryos of the recalcitrant seed species. Araucaria hunstenii K. Schum. J. of Plant Physiol. 37:1388-1397.
Pence, V.C. 1995. Cryopreservation of recalcitrant seeds, pp. 29-52. Biochemistry in agriculture and forestry Vol 32 Cryopreservation of Plant Germplasm I. Bajaj Y.P.S (Ed). Springer Verlag, Berlin.
Vertucci, C.W., Berjack .P., Pammentee N.W. and Cram, T. 1991. Cryopreservation of embryonic axes of an homeohydrous (recalcitrant) seed in relation to calorimetric properties of tissue water. Cryo-Letters 12:339-350.
Assy-Bah, B. and Engelmann, F. 1992b. Cryopreservation of immature embryos of coconut (Cocos nucifera L.) Cryo-Letters 13:67-74.
Chin, H.F., Krishnapillay, B. and Hor, Y.L. 1989. A note on the cryopreservation of embryos from young coconut (Coconut nucifera L.) var.Mawa. Pertanika 12: 183-186.
CPCRI, 2000. Annual Report - 2000. Central Plantation Crops Research Institute, Kasaragod. Kerala, India, pp.30-31.
Dumet, D., Engelmann, F., Chabrillange and Duval, Y. 1993. Cryopreservation of oil palm (Elaeis guineensis Jacq) somatic embryos involving a desiccation step. Plant Cell Reports: 12:352-355.
Engelmann, F. 1992. Cryopreservation of embryos, pp.281-290. In: Reproductive Biology and Plant Breeding, V. Dattee, C. Dumas and A. Gallais (Eds.) Springer Verlag Berlin.
Engelmann, F. 2000. Importance of cryopreservation for conservation of plant genetic resources. In: Cryopreservation of Tropical Plant Germplasm. Current Research Progress and Application. F. Engelmann and H. Takagi (Eds). JIRCAS, Japan/ IPGRI, Italy.
Gray, D.J., Conger, B.V, and Songstad, 1987. Desiccated quiescent somatic embryos of orchardgrass for use as synthetic seeds. In Vitro 23:29-33.
Grout, B.W.W , Shelton, K. and Pritchard, H.W. 1983. Orthodox behaviour of oil palm seed and cryopreservation of the excised embryo for genetic conservation Ann. Bot. 52:381-384.
Kim, Y.H and Janick, J. 1989 ABA and polyox-encapsulation of high humidity increases survival of desiccated somatic embryos of celery. Hort. Sci. 24:674-676.
Karun, A., Shivashankar, S., Sajini, K.K. and Saji, K.V. 1993 Field collection and in vitro germination of coconut embryos. J. Plant. Crops 21 (Suppl.): 291-294.
Karun, A., Sajini K.K. and Shivashankar, S. 1999. Embryo culture - A CPCRI protocol. Indian J. Horti. 56(4): 348-353.
Karun, A., Parthasarathy V.A., Kumaran P.M., Iyer, R.D. and Sajini, K.K. 2002. Coconut embryo culture - A protocol fpr germplasm collection Tech. Bull. 45 CPCRI, Kasaragod, India.
Niino, T. 1993. Cryopreservation of deciduous fruits and mulberry trees. In: Cryopreservation of Plant Genetic Resources A. Sakai (Ed) Ref No. 6 FARDA, Tokyo, pp.71-76.
Normah, M.N., Chin, H.F and Hor, Y.L. 1986. Desiccation and cryopreservation of embryonic axes of Hevea brasiliensis Mucl. Arg. Pertanika 9:299-303.
Pritchand, H.W. and Prendergast, F.G. 1986. Effect of desiccation on the in vitro viability of embryos of the recalcitrant seed species. Araucaria hunstenii K. Schum. J. of Plant Physiol. 37:1388-1397.
Pence, V.C. 1995. Cryopreservation of recalcitrant seeds, pp. 29-52. Biochemistry in agriculture and forestry Vol 32 Cryopreservation of Plant Germplasm I. Bajaj Y.P.S (Ed). Springer Verlag, Berlin.
Vertucci, C.W., Berjack .P., Pammentee N.W. and Cram, T. 1991. Cryopreservation of embryonic axes of an homeohydrous (recalcitrant) seed in relation to calorimetric properties of tissue water. Cryo-Letters 12:339-350.
Published
2005-06-01
How to Cite
Anitha Karun, K.K. Sajini, & V.A. Parthasarathy. (2005). Cryopreservation of mature coconut embryos by desiccation method. CORD, 21(01), 34. https://doi.org/10.37833/cord.v21i01.395
Section
Articles
