AN INVESTIGATION TOWARDS DEVELOPING A MOLECULAR APPROACH TO IMPROVE THE EFFICIENCY OF COCONUT BREEDING BY RAPD-MARKER ASSISTED SELECTION
Keywords:
Coconut Breeding, Developing a Molecular
Abstract
Tall, dwarf green and San Ramon are three phenotypically distinct forms of coconut, currently used for production of hybrid seeds in Sri Lanka. Development of molecular markers for further improvement of important economic traits of these hybrids was thought important due to various constraints faced by conventional breeders. Sixty random primers (OPERON) were used for generation of RAPD profiles using DNA from 3 individuals of each form. All the sixty primers except one (OPC3) yielded RAPD-PCR products with coconut DNA. They generated a total of 326 consistently amplified bands of which 54 distinguished tall, dwarf green and San Ramon. Among RAPDs detected 16 and 8 were specific to tall and dwarf respectively, 18 distinguished dwarf and San Ramon from tall, 9 distinguished tall and San Ramon from dwarf and 3 distinguished tall and dwarf green from San Ramon. It is expected to study the segregation of these markers in F2 populations derived from tall x dwarf green and tall x San Ramon F1 palms.
References
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RHODE,, W., KULLAYA, A., RORRIGUEZ, J., and RITTER, E. (1995). Genome analysis of Cocos nucifera L. by PCR amplification of spacer sequences separating a subset of copia-like EcoRI repetitive elements. Journal of Genetics and Breeding 49 : 179 - 186
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ROGGERS, S.O. and BENDICH, A. J. (1985). Extraction of DNA from milligram amounts of fresh, herbarium and mummified plant tissues. Plant Molecular Biology 5 : 69 – 76
SERIKAWA, T, KURAMATO, T, HILBERT, P., MORI, M., YAMADA, J, DAVEY, C.J., LINDPAINTER, K., GANTEN, D., GUENET, J.I., LATHROP, G.N. and BECKERMANN, J.S. (1992). Rat gene mapping using PCR analyzed micro-satellites. Genetics 131: 701 – 721
WEISING, K. and KARL, G. (1997). Hybridization-based micro-satellite fingerprinting of plants and fungi in DNA markers; protocols, applications and overviews. G. Caetano-Anolles and P.M. Gresshoff (eds.). pp. 27 – 53
WILLIAMS, J.G.K., KUBELIK, A.R., LIVAK, K.J., RAFALSKY, J.A. and TINGEY, S.V. (1991). DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Research 18: 6531
ZABEAU, M and VOSS, P. (1993). Selective restriction fragment length amplification: a general method for DNA fingerprinting. Eur. Pat. App. 92402629.7 (Pub no. 0 534858-A1)
BECKMANN, J.S. and SOLLER, M. (1983). Restriction fragment length polymorphisms in genetic improvement: methodologies, mapping and costs. Theoretical and Applied Genetics. 66; 35 -43
BOTSTEIN, D., WHITE, R.L., SKOLNICK, M.H. and DAVIS, R.W. (1980). Construction of a genetic map in man using restriction fragment length polymorphisms. American Journal of Human Genetics 32: 314 –331
DELLAPORTA, S.L, WOOD, J., and HICKS, J.B. (1983). A plant DNA mini-preparation: version II. Plant Molecular Biology Reporter 1: 19 - 21
DOYLE, J.J. and DOYLE, J.L (1990). Isolation of plant DNA from fresh tissue. Focus 12: 13 - 15
EVERARD, J.M.D.T. (1996a). Use of molecular markers for breeding of the coconut palm, Cocos nucifera. M.Sc. thesis, University of New England, NSW, Australia
EVERARD, J.M.D.T., KATZ, M and GREGG, K. (1996b), Inheritance of RAPD markers in the coconut palm, Cocos nucifera. Tropical Agricultural Research: 8: 124-134
EVERARD, J.M.D.T. and KATZ, M. (1998). Use of RAPDs for estimation of genetic distances between populations of the coconut palm, Cocos nucifera, L. Proceedings of the second Annual Forestry Symposium. University of Sri Jaywardenepura Sri Lanka.
FERNANDO, W.M.U. (1987). San Ramon big with promise. Coconut bulletin 4: 9 Harris. C. (1978). Evolution, dissemination and classification of Cocos nucifera, L.Botanical Review 44:265–320
LIYANAGE,, D.V. (1958). Varieties and forms of the coconut palm grown in Ceylon. Ceylon Coconut Quarterly 9; 1-10
LIYANAGE, D.V., WICKREMARATNE, M.R.T. and JAYASEKARA, C.J. (1988) Coconut breeding in Sri Lanka. Cocos 6: 1 - 26
MICHELMORE, R.W., PARAN, I. and KESSILI, R.V. (1991). Identification of markers linked to disease resistance by bulk segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proceedings of the national Academy of Science USA 88: 9828 –32
MULLIS, K., FALOON, S., SCHARF, S., SAKAI, R., HORA, G. and EDRICH, H. (1986). Specific enzymatic amplification of DNA in vitro. The polymerase chain reaction. Cold Springs Habour Symposium. Quant Biol. 51: 263-73
MURRAY, M.G. and THOMPSON, W.F. (1980). Rapid isolation of high molecular weight plant DNA. Nucleic Acids Research 8: 4321 – 4325
RHODE,, W., KULLAYA, A., RORRIGUEZ, J., and RITTER, E. (1995). Genome analysis of Cocos nucifera L. by PCR amplification of spacer sequences separating a subset of copia-like EcoRI repetitive elements. Journal of Genetics and Breeding 49 : 179 - 186
RHODE, W., SALAMANI, F., ASHBURNER, R. and RANDLES, J.W. (1992). An EcoRI repetitive sequence family of the coconut palm Cocos nucifera L. shows sequence homology to Copia-like elements. Journal of Genetics and Breeding 46; 391 –394
ROGGERS, S.O. and BENDICH, A. J. (1985). Extraction of DNA from milligram amounts of fresh, herbarium and mummified plant tissues. Plant Molecular Biology 5 : 69 – 76
SERIKAWA, T, KURAMATO, T, HILBERT, P., MORI, M., YAMADA, J, DAVEY, C.J., LINDPAINTER, K., GANTEN, D., GUENET, J.I., LATHROP, G.N. and BECKERMANN, J.S. (1992). Rat gene mapping using PCR analyzed micro-satellites. Genetics 131: 701 – 721
WEISING, K. and KARL, G. (1997). Hybridization-based micro-satellite fingerprinting of plants and fungi in DNA markers; protocols, applications and overviews. G. Caetano-Anolles and P.M. Gresshoff (eds.). pp. 27 – 53
WILLIAMS, J.G.K., KUBELIK, A.R., LIVAK, K.J., RAFALSKY, J.A. and TINGEY, S.V. (1991). DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Research 18: 6531
ZABEAU, M and VOSS, P. (1993). Selective restriction fragment length amplification: a general method for DNA fingerprinting. Eur. Pat. App. 92402629.7 (Pub no. 0 534858-A1)
Published
1999-06-01
How to Cite
J.M.D.T. Everard. (1999). AN INVESTIGATION TOWARDS DEVELOPING A MOLECULAR APPROACH TO IMPROVE THE EFFICIENCY OF COCONUT BREEDING BY RAPD-MARKER ASSISTED SELECTION. CORD, 15(02), 34. https://doi.org/10.37833/cord.v15i02.326
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Articles
