EFFECT OF DIFFERENT BRANDS OF ACTIVATED CARBON ON GROWTH AND DEVELOPMENT OF COCONUT (Cocos Nucifera L) EMBRYOS IN VITRO
Keywords:
Activated Carbon, Development of Coconut
Abstract
The effect of different brands of activated charcoal [Merck GR (Art. 2186), Sigma acid washed (C‑4386), Sigma neutralized (C‑3790), and Duchefa neutralized (C‑1302)] on growth and development ofcoconut zygotic embryos in vitro was evaluated. Analysis of data noted after one month revealed that there was no significant difference on percentage germination, shoot length, and number ofprimary root of cv. Laguna Tall embryos cultured in Y3 liquid medium supplemented with 2.5gll AC of different brands. Length of primary root of embryos cultured in Duchefa neutralized AC was significantly different from those in Sigma neutralized AC only after one month from initial culture. For the succeeding periods (2‑4 months), no significant difiference was observed among the treatments in terms of increment in plant height, number of scale and true leaves and length ofprimary root. Statistical analysis revealed thatpercentage ofseedlings with primary, secondary and tertiary roots did not differ significantly among the treatments 1‑4 months from initial culture. Results suggest that any brand (even alternately) can be used satisfactorily in the in vitro culture of coconut embryos.
References
ANAGNOSTAKIS, S.L. (1974). Haploid plants from anthers of tobacco enhancement with charcoal. Planta 115:281 283.
BON, M.C., GENDRAUD, M & FRANCLET, A (1988). Roles of phenolic compounds on micropropagation ofjuvenile and. mature clones of Sequoiadendron giganteum: Influence of activated charcoal. Sc. Hort.34:283 291.
COUTTON, M.E. & PREECE, J.E. (1986). Exudation and explant establishment. Newl. I.A.P.T.C. 50:9 18
DE GUMVIAN, E.V. & DEL ROSARIO, A.G. (1964).The growth and development of Cocos Nucifera L. Makapuno embryo in vitro. Phil. Agric. 48 (2 3)82 94
EBERT, A. & TAYLOR, H.F. (1990) Assessment of the changes of 2,4 dichlorophenoxyacetic acid concentrations in plant tissue culture media in the presence of activated charcoal. Plant Cell Tiss. Org. Cult. 20:165 172
EBERT, A. & TAYLOR, H.F. & BLAKE, J. (1993). Changes of 6-¬benzylaminopurine and 2,4 dichlorophenoxyacetic acid concentrations in plant tissue culture media in the presence of activated charcoal. Plant Cell Tiss. Org. Cult. 33:157 162
FRIDBORG, G.,. & ERIKSSON, T. (1975). Effects of activated charcoal on growth and morphogenesis. Physiol. Plant. 34:306¬308
FRIDBORG, G., PEDERS, M., LANDSTROM, L.E. & ERIKSOON, T (1978). The effect of activated charcoal on tissue cultures: adsorption of metabolites inhibiting morphogenesis. Physiol. Plant. 43:104 10
GEORGE, E.F. (l 993) Plant propagation by tissue culture (2nd 3 d.) Part l The Technology. Exegetics Ltd., Edington, Wilts. England. Pp. 470 471.
HORNER, M., McCONIB, J.A.McCONIB, A.J. & STREET, H.E. (1 977).Ethylene production and plantlet formation by Nicotiana anthers cultured in the presence and absence of charcoal. J. Exp. Bot. 28:1365 1372.
JOHANSSON, L. & ERIKSSON, T. (1977). Induced embryo formation in anther cultures of several, anemone species. Physiol. Plant. 44:172 174.
NAIRN, B.J. (1988). Significance of gelling agents in a production tissue culture laboratory. Comb. Proc. Int. Plant Prop. Soc. 1987. 37:200 205
NISSEN, S.J. & SUTTER, E.G. (1988). Stability of IAA and EBA in nutrient medium. after autoclaving and after storage under various environmental conditions. Hort Science 23:758.
NISSEN, S.J. & SUTTER, E.G. (1990). Stability of IAA and 113A in nutrient medium to several. tissue culture procedures. Hort Science 25:800 802.
NWANKO, B.A. & KRIKORIAN, A.D. (1983). Morphogenetic po¬tential of embryo and seedling derived callus of Elaeis guineensis Jacq. Var. pisifera Becc. Ann. Bot. 51:65 76
PARANJOTHY, K. & ROHANI, O. (1982) In vitro propagation of oil palm. In: Fujiwara, A. (Ed).pp. 747 749.
PECK, D.E. & CUMMING, B.G. (1986). Beneficial. effects of activated charcoal on bulblet production in tissue cultures of Muscari armeniacum. Plant Cell Tiss. Org. Cult. 6:9 14
PIERIK, R.L.M. (1987) In vitro culture of higher plants. Martinus Nijhoff Publishers, Dordrecht, Netherlands. 344 pp.
REBECHAULT, H. & MARTIN, J.P. (1976) Callus cultures. In: Paranjothy, K (ed) A Review of tissue culture of oil palm and other palms. PORIN Occasional. Paper, No. 3 July 1982.
REYNOLD, J.F. & MURASFHGE, T. (1979). Asexual embryogenesis in callus cultures of palms. In Vitro 15:383 387.
RILLO, E.P. & PALOMA, M.B. (1990). Comparison of three media formulations for in vitro culture of coco embryos. Oleagianeux 45 (7):319 323
RILLO, E.P. & PALOMA, M.B. (1992).In vitro culture of Makapuno coconut embryos. Coconuts Today 9(l):90 1 01
TISSERAT, B. (1979). Propagation of date palm (Phoenix dactylifera L.) in vitro. J. Exp. Bot. 30:1275 1283.
TISSERAT, B. (1984). Chapter 18 Date palm. In Sharp et al (eds) Propagation of date palm (Phoenix dactylVera L.) in vitro. J. Exp. Bot. 30: 1275 1283.
TYAGI, A.K., RASHD, A. & MAHESWARI, S.C. (1980). Enhance¬ment of pollen embryo formation in Datura innoxia by char¬coal. Physiol. Plant. 49:294 298
VENKETESWARAN, S. DIAS, M.A.D.L., SULTANBAWA, F. & WEYERS, U.V. (1988). Tissue culture studies on mahogany tree. Sweitenia. In: Ahuja NM (ed.) Somatic cell genetics of woody plants. Kluwer Acadernic Publishers, Dordrecht, The Netherlands. pp. 147 153.
WEATHERHEAD, M.A., BURDON, J. & HENSHAW, G.G. (1978). Some effects of activated charcoal as an additive to plant tissue culture media. Z. Pflansenphysiol. 94:399 406
WEATHERHEAD, M.A., BURDON, J. & BENSHAW, G.G.(1979). Effects of activated charcoal as, an additive to plant tissue culture media. Z. Pflansenphysiol. 94:399 406
ZAGHMOUT, O.M.F. & TORELLO, W.A. (1988). Enhanced regeneration in Iong term callus cultures of red fescue by pretreatment with activated charcoal. Hort Science 23:615 616.
BON, M.C., GENDRAUD, M & FRANCLET, A (1988). Roles of phenolic compounds on micropropagation ofjuvenile and. mature clones of Sequoiadendron giganteum: Influence of activated charcoal. Sc. Hort.34:283 291.
COUTTON, M.E. & PREECE, J.E. (1986). Exudation and explant establishment. Newl. I.A.P.T.C. 50:9 18
DE GUMVIAN, E.V. & DEL ROSARIO, A.G. (1964).The growth and development of Cocos Nucifera L. Makapuno embryo in vitro. Phil. Agric. 48 (2 3)82 94
EBERT, A. & TAYLOR, H.F. (1990) Assessment of the changes of 2,4 dichlorophenoxyacetic acid concentrations in plant tissue culture media in the presence of activated charcoal. Plant Cell Tiss. Org. Cult. 20:165 172
EBERT, A. & TAYLOR, H.F. & BLAKE, J. (1993). Changes of 6-¬benzylaminopurine and 2,4 dichlorophenoxyacetic acid concentrations in plant tissue culture media in the presence of activated charcoal. Plant Cell Tiss. Org. Cult. 33:157 162
FRIDBORG, G.,. & ERIKSSON, T. (1975). Effects of activated charcoal on growth and morphogenesis. Physiol. Plant. 34:306¬308
FRIDBORG, G., PEDERS, M., LANDSTROM, L.E. & ERIKSOON, T (1978). The effect of activated charcoal on tissue cultures: adsorption of metabolites inhibiting morphogenesis. Physiol. Plant. 43:104 10
GEORGE, E.F. (l 993) Plant propagation by tissue culture (2nd 3 d.) Part l The Technology. Exegetics Ltd., Edington, Wilts. England. Pp. 470 471.
HORNER, M., McCONIB, J.A.McCONIB, A.J. & STREET, H.E. (1 977).Ethylene production and plantlet formation by Nicotiana anthers cultured in the presence and absence of charcoal. J. Exp. Bot. 28:1365 1372.
JOHANSSON, L. & ERIKSSON, T. (1977). Induced embryo formation in anther cultures of several, anemone species. Physiol. Plant. 44:172 174.
NAIRN, B.J. (1988). Significance of gelling agents in a production tissue culture laboratory. Comb. Proc. Int. Plant Prop. Soc. 1987. 37:200 205
NISSEN, S.J. & SUTTER, E.G. (1988). Stability of IAA and EBA in nutrient medium. after autoclaving and after storage under various environmental conditions. Hort Science 23:758.
NISSEN, S.J. & SUTTER, E.G. (1990). Stability of IAA and 113A in nutrient medium to several. tissue culture procedures. Hort Science 25:800 802.
NWANKO, B.A. & KRIKORIAN, A.D. (1983). Morphogenetic po¬tential of embryo and seedling derived callus of Elaeis guineensis Jacq. Var. pisifera Becc. Ann. Bot. 51:65 76
PARANJOTHY, K. & ROHANI, O. (1982) In vitro propagation of oil palm. In: Fujiwara, A. (Ed).pp. 747 749.
PECK, D.E. & CUMMING, B.G. (1986). Beneficial. effects of activated charcoal on bulblet production in tissue cultures of Muscari armeniacum. Plant Cell Tiss. Org. Cult. 6:9 14
PIERIK, R.L.M. (1987) In vitro culture of higher plants. Martinus Nijhoff Publishers, Dordrecht, Netherlands. 344 pp.
REBECHAULT, H. & MARTIN, J.P. (1976) Callus cultures. In: Paranjothy, K (ed) A Review of tissue culture of oil palm and other palms. PORIN Occasional. Paper, No. 3 July 1982.
REYNOLD, J.F. & MURASFHGE, T. (1979). Asexual embryogenesis in callus cultures of palms. In Vitro 15:383 387.
RILLO, E.P. & PALOMA, M.B. (1990). Comparison of three media formulations for in vitro culture of coco embryos. Oleagianeux 45 (7):319 323
RILLO, E.P. & PALOMA, M.B. (1992).In vitro culture of Makapuno coconut embryos. Coconuts Today 9(l):90 1 01
TISSERAT, B. (1979). Propagation of date palm (Phoenix dactylifera L.) in vitro. J. Exp. Bot. 30:1275 1283.
TISSERAT, B. (1984). Chapter 18 Date palm. In Sharp et al (eds) Propagation of date palm (Phoenix dactylVera L.) in vitro. J. Exp. Bot. 30: 1275 1283.
TYAGI, A.K., RASHD, A. & MAHESWARI, S.C. (1980). Enhance¬ment of pollen embryo formation in Datura innoxia by char¬coal. Physiol. Plant. 49:294 298
VENKETESWARAN, S. DIAS, M.A.D.L., SULTANBAWA, F. & WEYERS, U.V. (1988). Tissue culture studies on mahogany tree. Sweitenia. In: Ahuja NM (ed.) Somatic cell genetics of woody plants. Kluwer Acadernic Publishers, Dordrecht, The Netherlands. pp. 147 153.
WEATHERHEAD, M.A., BURDON, J. & HENSHAW, G.G. (1978). Some effects of activated charcoal as an additive to plant tissue culture media. Z. Pflansenphysiol. 94:399 406
WEATHERHEAD, M.A., BURDON, J. & BENSHAW, G.G.(1979). Effects of activated charcoal as, an additive to plant tissue culture media. Z. Pflansenphysiol. 94:399 406
ZAGHMOUT, O.M.F. & TORELLO, W.A. (1988). Enhanced regeneration in Iong term callus cultures of red fescue by pretreatment with activated charcoal. Hort Science 23:615 616.
Published
1998-12-01
How to Cite
Zosimo S. Bonaobra III, Erlinda P. Rillo, & Osmundo D. Orense. (1998). EFFECT OF DIFFERENT BRANDS OF ACTIVATED CARBON ON GROWTH AND DEVELOPMENT OF COCONUT (Cocos Nucifera L) EMBRYOS IN VITRO. CORD, 14(02), 34. https://doi.org/10.37833/cord.v14i02.318
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Articles
