SHORT TERM in vitro PRESERVATION OF COCONUT SEED MATERIAL: A METHOD TO FACILITATE FIELD COLLECTION AND TRANSPORT OF COCONUT GERMPLASM

Abstract

High cost of transport and the short storage fife of recalcitrant seednuts are two major obstacles to the effective field collection and exchange of coconut germplasm. An in vitro method invol­ving the culture of zygotic embryos of coconut was developed to overcome these problems. The excised embryos were initially subjected to a slow growth phase by culturing in an agar based medium containing nutrients barely sufficient for their survival. After 2, 3 and 5 months in this medium, the embryos were trans­ferred to the gerinination or the development medium for resumption of growth and plant development. 53% of the zygotic embryos preserved in this manner for a period of 2 months produced nor­mal plants when transferred to the gennination medium. The percentage germination dropped to 32 after 5 months storage in the survival medium. The prolonged low temperature storage of cultures (10⁰ and 4⁰C for three months), caused irreversible damage to the coconut embryos and no plants were formed when transferred to the germination medium.

The advantages of the technique are that a large number of 'seednuts' can be transported free of pests in a small container and requires no change in the culture medium during the preser­vation period. Unlike the germination medium, the survival me­dium is agar based and hence the risk of containination is low. The non gerridnators can be identified and rejected prior to the germination phase.