Effect of Arbuscular Mycorrhizal Fungi Based Biofertilizer on Coconut Seedlings Growth in Nursery
Abstract
The success of a coconut plantation, in terms of its field establishment as well as its future performance, is heavily dependent on the quality of the seedlings used. Coconut nurseries adopt different agronomic practices to produce good quality seedlings. Applying mycorrhizal inoculants is becoming an increasingly common practice in plant nurseries as it facilitates healthy seedling growth resulting in healthy and vigorous seedlings in the nursery, helps to reduce mortality rates of seedlings in the field and reduce water and fertilizer consumption. In this study effects of mycorrhizae based biofertilizer were evaluated for the growth of coconut seedlings in the nursery and field. The experiment was conducted at the Makadura Research Center in Sri Lanka. The nursery experiment treatments were, the presence of AMF based biofertilizer (50g per seedling or per bag) and absence of mycorrhizae based biofertilizer. In the field experiment, five treatments with 500g of AMF biofertilizerwith 25% recommended inorganic fertilizer mixture for young coconut palms (YPM) per seedling, 500g of AMF biofertilizer with 50% YPM per seedling, 500g of AMF biofertilizer with 75% YPM per seedling, 500g of AMF biofertilizer with 100% YPM per seedling and with only YPM per seedling. Root and shoot growth of seedlings were measured at monthly intervals in the nursery experiment. Leaf production rate and stem girth of the seedling were measured at six months interval in the field experiment. The application of bio fertilizer increased the volume and dry weight of primary, secondary, tertiary and quaternary roots in seedlings in the nursery significantly. A significantly higher leaf production rate and stem girth was observed in the field seedlings treatment with 500g of AMF biofertilizer with 75% YPM. The experiment concluded that application of bio fertilizer is beneficial for coconut seedlings in the nursery for the production good quality seedlings with well-developed roots resulting in better field establishment and in the field for fast and vigorous growth.
References
Auge, R. M. 2001. Water relations, drought and vesicular-arbuscularmycorrhizalsymbiosis.Mycorrhiza, 11: 3-42.
Berta, G., Fusconi, A.andTrotta, A. 1993. VA mycorrhizal infection and the morphology and function of root systems.Journal of Environmental Botany 33: 159-173.
Burdette, A.M. 1979. A non-destructive method for measuring the volume of intact plant parts.Canadian Journal of Forest Research, 9: 120-122.
Castellano, M.A. and Molina, R. 1989. Mycorrhizae. In: Landis, T.D.; Tinus, R.W.; McDonald, S.E.; Barnett, J.P. The Container Tree Nursery Manual, Volume 5. Agric. Handbook. 674. Washington, DC: U.S. Department of Agriculture, Forest Service: 101-167.
Coyne, J. and Mark, H. 1999. Soil Microbiology: An Exploratory Approach. Delmar Publishers. Albany, New York.
Espeleta J. F., Eissenstat, D. M.and Graham, J. H. 1999. Citrus root responses to localized drying soil: a new approach to studying mycorrhizal effects on the roots of mature trees. Plant and Soil 206: 1-10.
Gemma, J.L.andKoske, R.E. 2006.Use of Mycorrhizae in Restoration of Hawaiian Habitats.Hawaii Conservation Alliance. [online] Available at http://www.rngr.net/ Publications /ctnm.[Accessed at May 17, 2006].
George, E. 2000.Nutrients uptake, ppg. 288-307. In Y. Kapulink and D.D.Douds.Jr. (eds).AbuscularMycirrhizas: Physiology and function.KulwerAcadamic publishers, Netherland.
Gianinazzi, S., Schuepp, H., Barea, J.M., and Haselwandter, K. 2002.Mycorrhizal Technology in Agriculture: From Genes to Bioproducts. Birkha¨user, Basel, pp. 296.
Jeffries, P.andBarea, J.M.2000.Arbuscularmycorrhiza key component of sustainable plant–soil ecosystems. In: Hock, B. (Ed.), TheMycota, Volume IX. Fungal Associations. Springer-Verlag, pp. 95–113.
Kemery, R. D. and Dana, M. N. 1995.Prairie Remnant Soil as a Source of MyorrhizalInoculums.Horticulture Science 30 (5):1015-1016.
Linderman, R.G. and Davis, E.A. 2004.Evaluation of Commercial Inorganic and Organic Fertilizer Effects on ArbuscularMycorrhizae Formed by Glomusintraradices.Horticulture Technology 14 (2) 196-202.
Mapa, R.B., Dassanayake, A.R. and Nayakekorale, H.B. 2005. Soils of the Intermediate zone of Sri Lanka: morphology, characterization and classification. Soil Science Society of Sri Lanka, Peradeniya, Sri Lanka.
Marx, D.H., Cordeia, C.E., Manul, S. B. and Ruehle, J.L. 1989. Ectomycorrhizal development on pine by Pisolithustinctorius in bare-root and container seeding nurseries. 1. Efficiency of various vegetative inoculums formulations. New Forests 3: 45-56.
Menon, K.P.V. and Pandalai, K.M. 1958. The coconut palm; A Monograph, Indian Central Coconut Committee, South India.
Miller, R.M. and Jastrow, J.D. 1992.The applicatio1991n of VA mycorrhizae to ecosystem restoration and reclamation. In: Allen, M.F. (Ed.), Mycorrhizal Functioning. Chapman & Hall, Ltd., London, England, pp. 438–467.
[SAS] Statistical Analysis Systems, 1999.SAS 1, STAT Users Guide, Release, 7.00 Cary, NC: Statistical Analysis Systems Institute, 1028.
Wu, Q. S., Zou, Y. N. , Xia, R. X. and Wang, M. Y.2007. Five Glomusspecies affect water relations of Citrus tangerine during drought stress. Experimental Botany, 48:147-154.
Wu, Q.S., Li, G.H. and Zou, Y. N. 2011.Roles of arbuscularmycorrhizalfungion growth and nutrient acquisition peach (PrunuspersicaL. BATSCH) seedlings ,The Journal of Animal & Plant Sciences, 21(4): 746-750.
Yao, Q., Zhu, H. H.and Chen, J. Z.2005.Growth responses and endogenous IAA and iPAs changes of litchi (Litchi chinensisSonn.) seedlings induced by arbuscularmycorrhizal fungal inoculation.Horticulture Science 105:145-151.
